No air conditioning. Minimum reached to 6 degrees celcius in winter and maximum reached to 42 degrees in summer.
Ambient air allowed freely to enter and go out of culture room without any control on particle count.
Protection given only from direct incident sunlight and direct contact with rain.
Diffuse sunlight allowed freely as source of illumination; no artificial lighting. Natural daylength used.
Achievement:
Culture growth with reasonable control on contamination
Excellent robust cultures in all seasons
If:
You do not get satisfactory control on contamination,
Satisfactory growth
do not conclude that this system is not useful on large scale. Subsequent pictures will give you equally good results in large scale production
We also:
did get massive contamination problems in the beginning and
had to investigate thoroughly how to avoid troubleshooting
had to overcome handling problems
device special techniques to improve throughput from workstations
All this does require in depth insight into tissue culture and intensive lab work.
We have already done it. You do not have to spend years to get to that stage. We offer all this Know-how under consultancy arrangement.